便攜式熒光蛋白激發(fā)光源用于觀察dsred的表達(dá)

近日聊城大學(xué)農(nóng)學(xué)院在《Agronomy》期刊上發(fā)表《Agrobacterium rhizogenes-Mediated Genetic Transformation and Establishment of CRISPR/Cas9 Genome-Editing Technology in Limonium bicolor》，文獻(xiàn)中實(shí)驗(yàn)使用了LUYOR-3415RG便攜式雙波長(zhǎng)熒光蛋白激發(fā)光源，用于觀察dsred在植物根系的表達(dá)。

上圖：左側(cè)為白光下的觀察效果，

右側(cè)F圖：在LUYOR-3415RG便攜式激發(fā)光源照射下左側(cè)的有明顯的dsred的紅色熒光，右側(cè)為沒有熒光表達(dá)。

文獻(xiàn)摘要：

Limonium bicolor is a perennial herbaceous plant belonging to the Plumbaginaceae family. It can be used as a dried flower or in cut flower arrangements and serves as a model recretohalophyte. Its genome sequencing has been recently completed. However, the research on L. bicolor is limited by the absence of a highly efficient genetic transformation system. In this study, we established a highly efficient Agrobacterium rhizogenes-mediated L. bicolor genetic transformation method. The transgenic hairy roots were induced from the hypocotyl of L. bicolor using A. rhizogenes strain K599 harboring pRdGa4Cas9 plasmid (which carries an expression cassette of 35S::DsRed2). The transgenic shoots were regenerated from hairy root segments (~0.1 cm diameter), and induction efficiency was achieved at . The transgenic shoots with 4–5 rosette leaves were directly planted into the soil to induce the transgenic roots. Therefore, transgenic plantlets were produced. The DsRed2 can be used as a reliable reporter gene in screening transgenic plantlets. Furthermore, we also established a CRISPR/Cas9 system in L. bicolor employing the A. rhizogenes-mediated genetic transformation approach. The highly efficient transformation method and CRIPSP/Cas9 system established will provide a valuable tool for functional genomics investigation and trait improvement in L. bicolor.

文獻(xiàn)地址：https://doi.org/10.3390/agronomy13092244   

美國(guó)路陽(yáng)生產(chǎn)的LUYOR-3415系列便攜式雙波長(zhǎng)熒光蛋白激發(fā)光源在中國(guó)有數(shù)千臺(tái)的銷售，每年都有大量文獻(xiàn)發(fā)布在 期刊上，LUYOR-3415便攜式激發(fā)光源能夠觀察gfp、egfp、bfp、yfp、rfp等各種熒光蛋白在植物葉片、種子、愈傷以及各種模式動(dòng)物上的表達(dá)。如果您對(duì)便攜式激發(fā)光源還不是很了解，我們提供樣機(jī)免費(fèi)試用。