312nm中波紫外燈LEB-280L用于白內(nèi)障疾病研究

哈爾濱醫(yī)科大學(xué)發(fā)表文獻(xiàn)《 Melatonin inhibits ferroptosis and delays age-related cataract by regulating SIRT6/p-Nrf2/GPX4 and SIRT6/NCOA4/FTH1 pathways 》，文獻(xiàn)中的實(shí)驗(yàn)使用了美國(guó)路陽(yáng)公司生產(chǎn)的312nm中波紫外燈LEB-280L，使用紫外燈LEB-280L輸出不同劑量的紫外線輻照細(xì)胞，用LEB-280L模擬陽(yáng)光中的紫外線，用來(lái)研究不同劑量的中波紫外線對(duì)細(xì)胞凋亡的影響。312nm中波紫外燈LEB-280L內(nèi)置了2根8w 312nm紫外線燈管，并安裝了紫外濾光片，能夠輸出純正的中波紫外線，沒(méi)有uva、uvc紫外線，并可以選購(gòu)臺(tái)式支架，滿足實(shí)驗(yàn)的長(zhǎng)時(shí)間輻照，具體紫外線燈LEB-280L產(chǎn)品介紹請(qǐng)瀏覽：《實(shí)驗(yàn)室用紫外線燈-LEB 系列手持式中波紫外線燈》。

請(qǐng)注意，操作紫外線燈請(qǐng)務(wù)必佩戴專業(yè)紫外線防護(hù)眼鏡！

文獻(xiàn)簡(jiǎn)介：

 Introduction 

Cataracts are the primary cause of preventable blindness globally. With the ageing of the world’s population, the proportion of age-related cataract (ARC) patients will increase in both developed and developing countries . Surgical treatment of cataracts is the most effective method in the clinic. However, there is also a risk of intraoperative and postoperative complications, such as posterior capsule opacification, intraocular inflammation and corneal edema [2]. Among many kinds of environmental stresses, such as ultraviolet (UV) radiation, diabetes, drug intake, and excessive smoking and alcohol consumption, UV radiation is the most important risk factor for ARCs . It has been shown that cortical and posterior subcapsular cataracts can be caused dose-dependently by consistent ocular ultraviolet B (UVB) radiation exposure [4]. The formation of cataracts induced by UVB begins with cumulative oxidative damage to human lens epithelial cells (HLECs) . During this process, the modes of cell death include apoptosis , necroptosis and pyroptosis.

. Materials 

Human lens epithelial cell line B-3 (B-3) was obtained from the American Type Culture Collection (ATCC Cat# CRL-11421, RRID: CVCL_6367), SRA01/04 and human embryonic kidney 293 T cell line (HEK-293 T) were purchased from Chinese Academy of Sciences (Shanghai, China). Foetal bovine serum (FBS) was purchased from Gibco (Thermo Fisher Scientific, Inc., Waltham, MA, USA), and Dulbecco’s modified Eagle’s medium was obtained from VivaCell Shanghai (Shanghai, China). UVB exposure was executed with a UVB radiometer (LUYOR-LEB-280L, USA) with a total output of 5 W/m2 (312 nm). A lux meter was used to test the irradiance of UV radiation. A Cell Counting Kit-8 (CCK-8) was purchased from Merck (Darmstadt, Germany). TRIzol and primers were obtained from Invitrogen (Eugene, Oregon, USA). Primary antibodies and fluorochrome-labeled secondary antibodies 

were purchased from Abcam (Cambridge, UK) and Proteintech (Chicago, IL, USA). All small molecular inhibitors and activators were obtained from MedChemExpress (Monmouth Junction, NJ, USA) and Cayman Chemical Company (Ann Arbor, MI, USA).

文獻(xiàn)地址：https://doi.org/10.1016/j.biopha.2022.114048

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