近日，浙江農(nóng)林大學(xué)在《springer link》發(fā)表文獻題為《Genomes of diverse Actinidia species provide insights into cis-regulatory motifs and genes associated with critical traits》，文獻中的實驗使用了LUYOR-3415RG便攜式雙波長熒光蛋白激發(fā)光源觀察GFP的熒光，使用LUYOR-3415RG激發(fā)光源分選陽性的毛狀根。

文獻摘要：

Root transformation and VC content measurement

Healthy, semi-lignified branches were selected for agro-infiltration using the following procedure. Briefly, A. rhizogenes strain K599 carrying the overexpressing plasmid was cultured overnight in liquid LB medium supplemented with appropriate antibiotics at 28 °C until the OD600 value reached 0.8–1.0. The cultures were centrifuged at 6000 g for 10 min at room temperature and then resuspended in infiltration buffer (0.05 M MES, 2 mM Na3PO4, 0.5% (w/v) D-glucose, and 0.1 mM acetosyringone) to a final OD600 of 0.8. Branches of A. valvata were collected and cut into the segments of 5–10 cm in length using sterilized pruning shears. The base of the stems was then immersed in the A. rhizogenes suspension and vacuum infiltrated for approximately 30 min under standard vacuum conditions. Subsequently, the branches were inserted into sterilized vermiculite and placed in the greenhouse for cultivation, maintaining a temperature of 26 °C with a light cycle of 16 h of illumination followed by 8 h of darkness. Approximately 2–4 weeks after agro-infiltration, the success of genetic transformation was assessed by detecting the fluorescence of hairy roots using a portable excitation lamp (Luyor-3415RG, Shanghai, China). The transgenic and wild-type roots were collected and homogenized for VC content measurement using the kit purchased from Suzhou Grace Biotechnology Co., Ltd., China. (Art. No. G0201F).

文獻地址：https://link.springer.com/article/10.1186/s12915-024-02002-z   

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